isotope
Ammonium chloride is an inorganic compound that usually exists in the form of white crystalline solid or powder. It consists of ammonium ions (NH4+) and chloride ions (Cl-), has high solubility, is easily soluble in water, and ionizes into ammonium ions and chloride ions in water.
Isotope labeling of ammonium chloride is a technique that changes the chemical properties of ammonium chloride by introducing specific isotopes (15N and/or 2H) into the molecule. Alfa Chemistry supplies high-quality stable isotope labeled ammonium chloride to support your research and needs in the fields of biomolecular NMR, metabolism, metabolomics, proteomics, etc. Our high quality stable isotope labeled ammonium chlorides are available to support your research and needs in the fields of biomolecular NMR, metabolism, metabolomics, proteomics, etc.
Ammonium Chloride-D4
The ammonium ion of ammonium chloride-D4 contains a lot of deuterium (²H), so this is great for studying hydrogen exchange reactions and other isotope labelling experiments.
Precautions
Ammonium chloride-D4 has certain hazards, including acute toxicity and eye irritation. It is mainly used in scientific research and is not used as a drug or household medicine.
Application examples
Ammonium chloride-D4 can be used as an isotope labeling and tracking chemical reaction source of hydrogen atoms (H).
As a solvent or internal standard in NMR analysis, ammonium chloride-D4 is also available.
Preparation method
Ammonium chloride-D4 can be made by reacting deuterium gas (D2) with hydrogen chloride (HCl). The end product of the reaction is a mixture of ammonium chloride-D4 and hydrogen chloride (HCl). Dissolved, purified and dried the mixture, then ammonium chloride-D4 product can be obtained.
Ammonium Chloride-15N
Ammonium chloride-15N is an abundant source of the stable isotope nitrogen-15, which can be manipulated to examine nitrogen metabolism, protein synthesis and biochemical reactions.
Application areas
- Biochemistry: The common applications of ammonium chloride-15N are in the structure and function analysis of proteins. Protein interaction, expression and position can all be measured by biological nuclear magnetic resonance (bio-NMR) imaging.
- Metabolism studies: By adding ammonium chloride-15N substrate into the experimental system, magnetic resonance imaging (MRS) technology can be used to monitor metabolites at different time points to analyze metabolic pathways.
- Agricultural applications: Ammonium chloride-15N is a tracer of fertilizer in agriculture to evaluate plant nutrient absorption of nitrogen fertilizer, and calculate best fertilizer application.
- Environmental & Ecology: We can monitor pollution and nitrogen cycles with ammonium chloride-15N in ecology.
Application examples
- In marine sediment research, ammonium chloride-15N is used to study the relationship between anaerobic ammonia oxidation and sulfate and iron reduction fuel nitrogen loss.
- In the study of microbial ammonia oxidizing communities, ammonium chloride-15N is used to analyze the differences in microbial responses to ocean warming.